Antibody-Hapten Recognition at the Surface of Functionalized Liposomes Studied by SPR: Steric Hindrance of Pegylated Phospholipids in Stealth Liposomes Prepared for Targeted Radionuclide Delivery
Analysis of MAb 734 binding with DSPC/Chol/DSPE-DTPA-indium/DSPE-PEG2000 liposomes via (1/Ms), (1/s), and (M). The amount of DSPE-PEG contained in PEGylated liposomes varied from 0% to 5%. Liposomes were immobilized on L1 chip. The length of PEG chain was fixed unchanged at 2000. The DTPA amount was fixed. The score of χ 2 (Chi2) is <5; it means that the model used adequately describes our data. All experiments were duplicated. , , and values are the average ± standard deviations.