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Journal of Drug Delivery
Volume 2011 (2011), Article ID 453619, 8 pages
Research Article

Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction

1Department of Cardiovascular Dynamics, Research Institute, National Cerebral and Cardiovascular Center, 5-7-1 Fujishiro-dai,Suita, Osaka 565-8565, Japan
2Department of Nursing Science, Taisei Gakuin University, Sakai 587-8555, Japan
3Division of Molecular Regenerative Medicine, Department of Biochemistry and Molecular Biology, Osaka University Graduate School of Medicine, Suita 565-0871, Japan
4Division of Stem Cell Regulation Research, G6, Osaka University School of Medicine, Suita 565-0871, Japan
5Division of Hypertension, National Cerebral and Cardiovascular Center, Suita 565-8565, Japan

Received 6 August 2010; Accepted 31 October 2010

Academic Editor: Hasan Uludaǧ

Copyright © 2011 Kazuo Komamura et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We transfected naked HGF plasmid DNA into cultured cardiomyocytes using a sonoporation method consisting of ultrasound-triggered bubble liposome destruction. We examined the effects on transfection efficiency of three concentrations of bubble liposome ( , , /mL), three concentrations of HGF DNA (60, 120, 180 μg/mL), two insonification times (30, 60 sec), and three incubation times (15, 60, 120 min). We found that low concentrations of bubble liposome and low concentrations of DNA provided the largest amount of the HGF protein expression by the sonoporated cardiomyocytes. Variation of insonification and incubation times did not affect the amount of product. Following insonification, cardiomyocytes showed cellular injury, as determined by a dye exclusion test. The extent of injury was most severe with the highest concentration of bubble liposome. In conclusion, there are some trade-offs between gene transfection efficiency and cellular injury using ultrasound-triggered bubble liposome destruction as a method for gene transfection.