Cellular Injury of Cardiomyocytes during Hepatocyte Growth Factor Gene Transfection with Ultrasound-Triggered Bubble Liposome Destruction
Figure 4
(a) Effect of insonification time on cell viability using 60 μg of DNA, particles/mL liposome, and 15-min incubation with DNA, and three 30- or 60-sec insonifications. “US alone” represents the percentage of dead cells immediately after three 30-sec insonifications in the absence of liposome and DNA. * versus baseline. (b) Effect of liposome concentration on cell viability using 60 μg of DNA and three 30-sec insonifications and 15-min incubation with DNA. * versus baseline; ** versus particles/mL; *** versus particles/mL. (c) Effect of repetitions of insonification on cell viability using 60 μg of DNA, particles/mL liposome, and 15-min incubation with DNA. * versus baseline; ** versus 30 sec × 1.
