In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes
Figure 4
Effect of γ-CyDs on Renilla luciferase mRNA levels after transfection of pDNA/AF-liposomes or pDNA/γ-CyDs/AF-liposomes in HepG2 cells. The charge ratio of AF-liposomes/pDNA was 1.6. CyDs were added to AF-liposomes suspension before freeze-drying. The concentration of CyD was 1 μM/μM lipids. Cells were incubated with pDNA/γ-CyDs/AF-liposomes for 3 h in FCS-free medium. After washing twice, the cells were incubated for 21 h in culture medium supplemented with 10% FCS. The Renilla luciferase mRNA level in HepG2 cells was assayed by RT-PCR. The luciferase mRNA level in each sample was normalized to abundance of β-actin mRNA. Each value represents the mean ± SEM of 3–7 experiments. * versus without CyD.
