In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes
Figure 5
Gene transfer efficiency of pDNA/AF-liposomes or pDNA/γ-CyDs/AF-liposomes in HepG2 cells. pDNA was (a) pEGFP-N1 or (b) pGL3-control vector. The charge ratio of AF-liposomes/pDNA was 1.6. γ-CyDs were added to AF-liposomes suspension before freeze-drying. The concentration of CyDs was 1 μM/μM lipids. Cells were incubated with pDNA/γ-CyDs/AF-liposomes for 3 h in FCS-free medium. After washing twice, the cells were incubated for 21 h in culture medium supplemented with 10% FCS. (a) Cells were determined by a confocal laser scanning microscopy. The percentage in parenthesis represents frequency rate of EGFP expression cells. (b) The luciferase activity in cell lysates was determined using a luminometer. Each value represents the mean ± SEM of 3–7 experiments. * versus without CyD.
