Research Article
In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes
Table 3
Encapsulation ratios of pDNA and -CyDs into AF-liposomes.
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The charge ratio of AF-liposome/pDNA was 1.6. γ-CyDs were added to AF-liposome suspension before freeze-drying. The concentrations of γ-CyDs were 1 μM/μM lipids. The encapsulation ratios of pDNA were determined using Picogreen assay. The encapsulation ratios of γ-CyDs were determined by an anthrone-sulfuric acid method. Each value represents the mean ± SEM of 3 experiments. * versus without CyD. |