Research Article

In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes

Table 3

Encapsulation ratios of pDNA and -CyDs into AF-liposomes.

CyDEncapsulation ratio of pDNA (%)Encapsulation ratio of γ-CyDs (%)

Without CyD42.4 4.3
γ-CyD58.2 1.8 10.2 0.5
HP-γ-CyD41.9 3.311.0 2.2

The charge ratio of AF-liposome/pDNA was 1.6. γ-CyDs were added to AF-liposome suspension before freeze-drying. The concentrations of γ-CyDs were 1 μM/μM lipids. The encapsulation ratios of pDNA were determined using Picogreen assay. The encapsulation ratios of γ-CyDs were determined by an anthrone-sulfuric acid method. Each value represents the mean ± SEM of 3 experiments. *   versus without CyD.