Research Article
In Vitro Gene Delivery Mediated by Asialofetuin-Appended Cationic Liposomes Associated with γ-Cyclodextrin into Hepatocytes
Table 4
Encapsulation ratio of pDNA into AF-liposomes and gene transfer activity of pDNA/AF-liposomes at various cycles of freeze-thaw in HepG2 cells.
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The charge ratio of AF-liposomes/pDNA was 1.6. pDNA/AF-liposome was prepared using a freeze-thaw method which was repeated from 0 to 5 cycles. Cells were incubated with pDNA/γ-CyDs/AF-liposome for 3 h in FCS-free medium. After washing twice, the cells were incubated for 21 h in culture medium supplemented with 10% FCS. The luciferase activity in cell lysates was determined using a luminometer. Each value represents the mean SEM of 3 experiments. |