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Journal of Drug Delivery
Volume 2011 (2011), Article ID 647631, 7 pages
http://dx.doi.org/10.1155/2011/647631
Research Article

Preparation of DNA/Gold Nanoparticle Encapsulated in Calcium Phosphate

1Research Institute of Pharmaceutical Sciences, Musashino University, Shinmachi 1-1-20, Nishitokyo-Shi, Tokyo 202-8585, Japan
2Graduate School of Pharmaceutical Sciences, Tokyo University of Science, 2641 Yamazaki, Noda-Shi, Chiba-Ken 278-8510, Japan

Received 27 October 2010; Revised 4 March 2011; Accepted 5 April 2011

Academic Editor: Guru V. Betageri

Copyright © 2011 Tomoko Ito et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Biocompatible DNA/gold nanoparticle complex with a protective calcium phosphate (CaP) coating was prepared by incubating DNA/gold nanoparticle complex coated by hyaluronic acid in SBF (simulated body fluid) with a Ca concentration above 2 mM. The CaP-coated DNA complex was revealed to have high compatibility with cells and resistance against enzymatic degradation. By immersion in acetate buffer (pH 4.5), the CaP capsule released the contained DNA complex. This CaP capsule including a DNA complex is promising as a sustained-release system of DNA complexes for gene therapy.