Research Article
Effects of Atelocollagen Formulation Containing Oligonucleotide on Endothelial Permeability
Table 1
Effects of formulation composition on relative TER (%).
| (a) Effects of oligonucleotide type. |
| | Oligonucleotides | Atelocollagen concentration | TER (%) | | Type, sequences | Reference | Concentration |
| | Blank control | | | | | 100.0 | | Phosphorothioate Oligodeoxynucleotide | 5′-TGCATCCCCCAGGCCACCAT-3′ | 25 | 5 μM | | 92.7 | | Atelocollagen alone | | | | 0.1% | 88.1 | | Phosphorothioate Oligodeoxynucleotide | 5′-TGCATCCCCCAGGCCACCAT-3′ | 25 | 5 μM | 0.1% | 45.7 | | Phosphodiester Oligodeoxynucleotide | 5′-TGCATCCCCCAGGCCACCAT-3′ | | 5 μM | 0.1% | 81.4 | | Double stranded small RNA | 5′-UGCAUCCCCCAGGCCACCAUdTdT-3′ and complemental sequence | 20 | 5 μM | 0.1% | 57.9 |
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| (b) Effects of ODN sequence on TER (%). |
| | Oligonucleotides | | Atelocollagen | | Name | Sequence | Reference | Concentration | 0% | 0.1% |
| | | | | TER (%) | | Blank control | | | | 100 | — | | ODN-(1) | 5′-TGCATCCCCCAGGCCACCAT-3′ | 25 | 5 μM | 100 | 58 | | ODN-(2) | 5′-TCGCATCGACCCGCCCACTA-3′ | 25 | 5 μM | 100 | 54 | | ODN-(3) | 5′-GCTGATTAGAGAGAGGTCCC-3′ | 26 | 5 μM | 100 | 50 | | ODN-(4) | 5′-CCCTGGAGAGAGATTAGTCG-3′ | | 5 μM | 96 | 65 |
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| (c) Effects of ODN length on TER (%). |
| | Oligonucleotides | Atelocollagen | | Sequence | Concentration | 0% | 0.1% |
| | Examination (1) | | TER (%) | | Blank control | — | 94.6 | — | | Atelocollagen alone | — | — | 94.6 | | ODN 10-mer (5′-TGCATCCCCC-3′) | 5 μM | 106.3 | 102.8 | | ODN 15-mer (5′-TGCATCCCCCAGGCC-3′) | 5 μM | 94.1 | 47.1 | | ODN 20-mer (5′-TGCATCCCCCAGGCCACCAT-3′; Ref. [25]) | 5 μM | 107.1 | 50.0 | | ODN 30-mer (20-mer ODN + 10-mer ODN) | 5 μM | 102.9 | 43.8 |
| | Examination (2) | | TER (%) | | Blank control | — | 87.4 | — | | Atelocollagen alone | — | — | 83.3 | | ODN 20-mer (5′-TGCATCCCCCAGGCCACCAT-3′; Ref. [25]) | 5 μM | 91.7 | 51.4 | | ODN 30-mer (20-mer ODN + 10-mer ODN) | 5 μM | 83.3 | 26.5 | | ODN 50-mer (20-mer ODN × 2 + 10-mer ODN) | 5 μM | 85.7 | 35.3 | | ODN 100-mer (20-mer ODN × 5) | 5 μM | 82.4 | 50.0 |
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| (d) Effects of the concentrations of ODN and atelocollagen on TER (%). |
| | ODN concentration | Atelocollagen | | 0% | 0.003% | 0.03% | 0.1% |
| | TER (%) | | Blank control | 94.6 | | | | | 0.5 μM | 100.0 | | | 77.8 | | 5 μM | 102.8 | | | 43.7 | | 50 μM | 102.9 | | | 50.8 | | 500 μM | 91.7 | | | 28.5 |
| | TER (%) | | Atelocollagen alone | | | | 100.0 | | 5 μM | 94.9 | 98.1 | 54.9 | 52.0 |
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ODN sequence: 5′-TGCATCCCCCAGGCCACCAT-3′.
TER(%): relative value at 1.5 hrs compared to the value observed at the start of the experiment.
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