Cyclodextrin-Containing Polymers: Versatile Platforms of Drug Delivery Materials
Figure 8
Effect of AD-PEG-Tf incorporation on nanoparticle size, salt stability, and transgene efficiency. (a) Dynamic light scattering (DLS) measurements of nanoparticle size as a function of time after the addition of salt (phosphate-buffered saline) help to define an optimal formulation window above which excessive AD-PEG-Tf leads to salt-induced nanoparticles aggregation. Nanoparticles were prepared containing 0% (Ad-PEG) or the indicated mol% of AD-PEG-Tf (percentage of total cyclodextrins by mole, with the remaining balance to 100% comprised of AD-PEG). (b) When plasmid-containing nanoparticles are exposed to cultured cells, inclusion of AD-PEG-Tf in the formulation increases transgene expression in a manner that can be reversed by addition of soluble Tf as a competitor, suggesting that TfR-mediated endocytosis plays a role in nanoparticle uptake and/or intracellular trafficking. Treatments included cell alone (cells), non-AD-PEG-Tf-containing nanoparticles (PEG-part.), non-AD-PEG-Tf-containing nanoparticles plus 0.05 mol% free soluble Tf (PEG-part. ), AD-PEG-Tf-containing nanoparticles (Tf-PEG-part.), non-AD-PEG-Tf-containing nanoparticles plus 10 equivalents of free soluble Tf (PEG-part. ), or AD-PEG-Tf-containing nanoparticles plus 10 equivalents of free soluble Tf (Tf-PEG-part. ) (from [22]).
