Lipoplatin or DPPG-liposomes with fluorescent lipids enter rapidly MCF-7 breast cancer cells in culture. Time-course processing of FITC-labeled DPPG-containing liposomes (a) and Lipoplatin (b) using confocal microscopy. At 5 min, the majority of the signal is localized in the membrane. Lipids are rapidly internalized and at 4–24 hours, a strong signal is observed in the cytoplasm and at the perinuclear area. These results demonstrate that lipoplatin or DPPG-liposomes nanoparticles are able to cross the cell membrane barrier [20].
