PLGA Nanoparticles for Ultrasound-Mediated Gene Delivery to Solid Tumors
Targeted nanoparticles are promising for future in vivo gene delivery approaches. (a) PSMA-targeted PLGA-based microparticles enter LNCaP (PSMA+) PCa cells. Untreated control (1), after 30 min of exposure to nontargeted FITC-loaded (2), and targeted FITC-loaded (3) MBs. Cell nuclei were stained with Hoechst (blue). The number of green-positive cells per field was significantly different from that of nontargeted MBs. Reprinted from  with permission from American Chemical Society. (b) Confocal fluorescent scanning microscopy images detecting cellular uptake of MUC-1 targeted Aptamer conjugated NPs (top row) or NPs (bottom row) in MCF-7 cells. Green fluorescent FITC was encapsulated in Apt-NPs and NPs. The nuclei were stained blue with DAPI. The right column showed the merged images of the FITC and the DAPI channels. MCF-7 cells were exposed to FITC-encapsulated Apt-NPs or NPs at 100 μg/mL for 2 hours. Reprinted from  under the terms of the Creative Commons Attribution License.