Enhanced Dendritic Cell-Mediated Antigen-Specific CD4+ T Cell Responses: IFN-Gamma Aids TLR Stimulation

Figure 2

IFN-gamma enhances DC maturation with or without TLR ligands in vivo. C57BL/6 mice were injected with LPS (TLR4 ligand) or CpG (TLR9 ligand) with (solid line) or without (dotted line) IFN-gamma intradermally using the Hock immunization protocol. At 18 h, popliteal lymph cells were isolated. Live CD11c-high cells were analyzed for CD80 and CD86 expression. Data shown are representative of at least two experiments. The shaded area represents cells stained with the respective secondary antibody.