Development of Dorzolamide Loaded 6-O-Carboxymethyl Chitosan Nanoparticles for Open Angle Glaucoma

Table 8

Effect of drug loading on OCM-CS : CaCl₂ weight ratio of 6 : 1.

DRZ loading (%)	PS (nm)	PI	Zeta potential (mV)	EE of OCM-CSNs
Blank OCM-CSNs	174.0±1.29	0.269±0.021	−32.57±0.57	—
10%	181.1±2.19	0.209±0.081	−31.24±0.75	25.29±0.56
20%	187.1±2.72	0.219±0.006	−30.87±0.86	34.87±0.33
50%	212.4±3.21	0.244±0.016	−28.57±0.51	18.13±0.47
75%	239.6±3.81	0.316±0.029	−24.03±0.68	18.38±0.29

Values are expressed as mean ± standard deviation, 𝑛=3.
OCM-CS: 6-𝑂-carboxymethyl chitosan; CaCl₂: calcium chloride; PS: particle size; NPs: nanoparticles; PI: polydispersity index; EE: entrapment efficiency; OCM-CSNs: 6-𝑂-carboxymethyl chitosan nanoparticles; DRZ: dorzolamide hydrochloride.