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Journal of Drug Delivery
Volume 2015, Article ID 790480, 8 pages
Research Article

Dosing-Time Dependent Effects of Sodium Nitroprusside on Cerebral, Renal, and Hepatic Catalase Activity in Mice

1UMR Biosurveillance et Toxicologie Environnementale, Département de Biologie, Faculté des Sciences et Techniques de Maradi, 465 Maradi, Niger
2Circadian Rhythm Laboratory, Boise State University, 1910 University Drive, Boise, ID 83725, USA
3UR Ethnobotanie et Stress Oxydant, Département des Sciences de la Vie, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia
4National Dairy Research Institute, Eastern Regional Station, A-12, Kalyani,West Bengal 741235, India
5Laboratoire de Pharmacologie, Faculté de Médecine, 5019 Monastir, Tunisia
6Laboratoire de Biosurveillance de l’Environnement, Faculté des Sciences de Bizerte, 7021 Zarzouna, Tunisia

Received 2 December 2014; Revised 9 February 2015; Accepted 19 February 2015

Academic Editor: A. Fadda

Copyright © 2015 Mamane Sani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


To investigate the time dependence of sodium nitroprusside- (NPS-) induced oxidative effects, the authors study the variation of the antioxidant enzyme CAT activity in various tissues after the administration of a single 2.5 mg/kg dose of SNP or sodium chloride (NaCl 0.9%). For each of the two dosing times (1 and 13 hours after light onset, HALO, which correspond to the beginning of diurnal rest span and of nocturnal activity span of mice, resp.), brain, kidney, and liver tissues were excised from animals at 0, 1, 3, 6, 9, 12, 24, and 36 h following the drug administration and CAT activity was assayed. The results suggest that SNP-induced stimulation of CAT activity is greater in all three tissues when the drug is administered at 1 HALO than at 13 HALO. Two-way ANOVA revealed that CAT activity significantly () varied as a function of the sampling time but not of the treatment in all three tissues. Moreover, a statistically significant () interaction between the organ sampling-time and the SNP treatment was revealed in kidney regardless of the dosing time, whereas a highly significant () interaction was validated in liver only in animals injected at 13 HALO.