In Vivo siRNA Delivery and Rebound of Renal LRP2 in Mice

Figure 2

LRP2 mRNA expression in mS1PT cells is reduced. (a) Knockdown persists for at least 96 h and reaches baseline expression by 120 h ( cell culture experiments). Expression is increased as compared to scrambled (siSCR) expression at 144 h. (b) Megalin immunoblot reveals decreased protein expression in S1 proximal tubular cells treated with siLRP2. (c) Expression was reduced to 22.4% 24 h after siLRP2 administration and 9.1% 48 h after siLRP2 administration ( cell culture experiments). Densitometry was normalized to a beta-actin control. as compared to the siSCR control.

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