Pancreatic Beta-Cell Purification by Altering FAD and NAD(P)H Metabolism
Figure 6
Changes in cellular NAD(P)H fluorescence.
Beta-cell
(black bars) and nonbeta-cell (dashed bars) NAD(P)H fluorescence were measured
in response to (a) increasing concentrations of D-glucose and (b) amino acids.
Fluorescence is represented as relative NAD(P)H fluorescence, compared to
medium containing 553 mg/L amino acids and 5.5 mM D-Glucose. The percentage of high
NAD(P)H fluorescent cells in response to (c) increasing concentrations of
D-glucose and (d) amino acids was measured within the FAD fluorescent beta-cell
population. Black bars represent the percentage of high NAD(P)H fluorescent
cells within the FAD fluorescent beta-cell population. The dotted bars
represent the low NAD(P)H fluorescent cells within the FAD fluorescent beta-cell
population. The graphs represent four separate experiments, bars represent the
mean ± SEM, and statistical significance was calculated using the Mann-Whitney
test. -values <.05 were considered to be statistically significant and are
represented by (∗).
