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Experimental Diabetes Research
Volume 2008, Article ID 818341, 8 pages
Research Article

Change in Long-Spacing Collagen in Descemet's Membrane of Diabetic Goto-Kakizaki Rats and Its Suppression by Antidiabetic Agents

1Department of Anatomy, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan
2Department of Anatomy, Yokohama City University School of Medicine, Kanazawa Ku, Fukuura 3-9, Yokohama, Kanagawa 236-0004, Japan
3Department of Biochemistry, Kyorin University School of Medicine, Mitaka, Tokyo 181-8611, Japan

Received 24 January 2008; Revised 23 April 2008; Accepted 1 July 2008

Academic Editor: Timothy Kern

Copyright © 2008 Yoshihiro Akimoto et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We examined changes in the ultrastructure and localization of major extracellular matrix components, including 5 types of collagen (type I, III, IV, VI, and VIII), laminin, fibronectin, and heparan sulfate proteoglycan in Descemet's membrane of the cornea of diabetic GK rats. In the cornea of diabetic GK rats, more long-spacing collagen fibrils were observed in Descemet's membrane than in the membrane of the nondiabetic Wistar rats. Both GK and Wistar rats showed an age-dependent increase in the density of the long-spacing collagen. Immunoelectron microscopy showed that type VIII collagen was localized in the internodal region of the long-spacing collagen, which was not labelled by any of the other antibodies used. The antidiabetic agents nateglinide and glibenclamide significantly suppressed the formation of the long-spacing collagen in the diabetic rats. Long-spacing collagen would thus be a useful indicator for studying diabetic changes in the cornea and the effect of antidiabetic agents.