Identification of Compounds That Inhibit IGF-I Signaling in Hyperglycemia
Table 2
Effect of compounds on IAP cleavage, IAP-SHPS-1 association and IGF-I-stimulated signaling events.
Compound number
Decrease in R569 binding*
IAP-SHPS-1 association**
SHPS-1 phosphor**
SHPS-1-Shc association**
Shc phosphor**
ERK Phosphor**
1
97 ±2
80 ±5
87.5 ±12
96.5 ±3
76 ±8
93 ±5
2
91 ±1.5
97 ±2
93 ±7
95.5 ±4
77 ±13
84 ±14
3
85 ±3
100 ±0
99 ±1
89 ±0.5
81 ±10
84 ±9
4
63 ±18
58 ±15
94 ±5
85 ±5
65 ±10
79 ±13
5
80 ±6
52 ±1
77 ±14
90 ±2
83 ±4
70 ±13
6
78 ±6
55 ±10
90 ±5
93 ±1.5
85 ±7
65 ±15
7
83 ±3
92 ±1
76 ±14
94 ±3
80 ±20
85 ±12
8
78 ±1
55 ±5
76 ±7
84 ±7
66 ±16
92 ±1
9
70 ±8
21 ±2
50 ±28
84 ±6
90 ±5
73 ±12
10
78 ±2.5
90 ±8
85 ±8
86 ±5
57 ±7
82 ±8
11
50 ±5
60 ±12
90 ±4
75 ±10
65 ±15
90 ±4
12
78 ±4.5
55 ±5
89 ±11
78 ±2
78 ±2
99 ±1
13
45 ±5
97 ±1
90 ±10
84 ±3
73 ±4
89 ±4
14
72 ±4
66 ±7
47 ±5
67 ±2.5
77 ±3
92 ±1
% Decrease in R569 antibody binding compared with SMC maintained in 25 mM glucose. % Decrease in association, phosphorylation or recruitment in response to IGF-I compared with SMC maintained in 25 mM glucose. Results shown are mean ± sem .
