Murine Pancreatic Beta TC3 Cells Show Greater 2^′,5^′-Oligoadenylate Synthetase (2^′5^′AS) Antiviral Enzyme Activity and Apoptosis Following IFN-𝛼 or Poly(I:C) Treatment than Pancreatic Alpha TC3 Cells

Figure 7

Induction of apoptosis in beta TC3 and alpha TC3 cells after transfection of IFN-α4-containing vector and poly(I:C). After beta TC3 and alpha TC3 cells were transiently transfected with pcDNA3.1-mIFN-α4, poly(I:C), and pcDNA3.1-mIFN-α4 plus poly(I:C) for 24 hours, respectively, induction of apoptosis was detected by immunoblots. pcDNA3.1 empty vector was used as a negative control. Cells lysates were separated on 15% SDS-PAGE and transferred to nitrocellulose membranes. The polyclonal antibody specific to mouse caspase 3 was used to detect both the intact as well as the cleaved caspase 3 proteins.