Research Article

Sequence Variation and Expression of the Gimap Gene Family in the BB Rat

Table 1

Probes and primers used in qRT-PCR.

Primer namePrimer sequence to

Gimap8-f CCAGGAGACCCAGGTGAAAG
Gimap8-r AGTTGAATGCTCATCATAGCTCCTT
Gimap8-p6FAM-TCTGTTGACAATAGCCAATGATCTCA-BHQ1

Gimap9-f AGGAACGGCAGAGCCTACTTT
Gimap9-r CCACTAGACATTGGTTCAGCTTCTTA
Gimap9-p6FAM-CTGACAGGATATATAAGGACA-MGBQ

Gimap4-f AACATGCCGTACAGAGCTCACA
Gimap4-r AGTGGCACCATTAGAAGGCAAA
Gimap4-p6FAM-CCATGACACACCCACTCCAACAGGG-BHQ1

Gimap6-f TGGATGCTCTGGATGTTGCA
Gimap6-r TCCTGCTCATCCCCTTGTG
Gimap6-p6FAM-TTGTTGAAGCCACAATGGCGTCTCTCA-BHQ1

Gimap7-f GGACTCAGTGTCAGGCTCCAA
Gimap7-r CGGGAGGACAGGCTAGCATA
Gimap7-p6FAM-CTGGATCACACTTGGCGCTCAGCTC-BHQ1

Gimap1-f AGAGGCGGACCAGGTTCCTA
Gimap1-r CCTCCAGCCCTGCCTGTAG
Gimap1-p6FAM-TTCTGCCATCTCCACAGCCCA-BHQ1

Gimap5-f CATGTTAGGGAAGCTCAGTC
Gimap5-r GAAGGGTTCTACTGTGTCTCA
Gimap5-p6FAM-TTTCACTATCATTTGACTCCTGTGCA-BHQ1

Gimap3-f CCACAGGGAGTGTAGACCTTGAA
Gimap3-r CTGCTGTTTCCGAATCCAGTTT
Gimap3-p6FAM-ATCCTCCAGCGTCCAC-MGBQ

Lr8-f GCCTCTGGTTGTGCCTTCTG
Lr8-r CCCTGTCCCATCTCATGGAT
Lr8-p6FAM-CCCACTCCAGCCAAAATTGCCACA-BHQ1

Cyc-f CACCGTGTTCTTCGACAT
Cyc-r TTTCTGCTGTCTTTGGAACT
Cyc-pHEX-CTGCTTCGAGCTGTTTGCAGAC-BHQ1

Probes and primers were designed to bind near the end of the transcripts. f is forward primer, r is reverse primer, p is probe, 6FAM is 6-carboxyfluorescein, HEX is hexachlorofluorescein, and BHQ1 is black hole quencher 1.