Research Article

Rosiglitazone Prevents High Glucose-Induced Vascular Endothelial Growth Factor and Collagen IV Expression in Cultured Mesangial Cells

Figure 2

Effect of rosiglitazone on reactive oxygen species generation. Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) D-glucose for up to 48 hours, and pretreated with 10  M rosiglitazone (RSG) or GW9662 for 1 hour. Reactive oxygen species (ROS) were detected in DCF-loaded cells by confocal fluorescence imaging in 3 or 4 separate experiments for each condition. (a) ROS generation was inhibited by RSG ( cells, versus NG, versus HG 3 hours, HG 24 hours, or HG 48 hours). (b) ROS generation in NG was stimulated with GW9662 ( cells, versus NG, versus HG 48 hours without RSG or GW9662). Magnification bar = 25  M.