Rosiglitazone Prevents High Glucose-Induced Vascular Endothelial Growth Factor and Collagen IV Expression in Cultured Mesangial Cells
Effect of rosiglitazone on reactive oxygen species generation. Mesangial cells were exposed to 5.6 mM (NG) or 25 mM (HG) D-glucose for up to 48 hours, and pretreated with 10 M rosiglitazone (RSG) or GW9662 for 1 hour. Reactive oxygen species (ROS) were detected in DCF-loaded cells by confocal fluorescence imaging in 3 or 4 separate experiments for each condition. (a) ROS generation was inhibited by RSG ( cells, versus NG, versus HG 3 hours, HG 24 hours, or HG 48 hours). (b) ROS generation in NG was stimulated with GW9662 ( cells, versus NG, versus HG 48 hours without RSG or GW9662). Magnification bar = 25 M.