Figure 3: Macrophages in wound tissue. (a) Expression of lysozyme M mRNA in nonwounded back skin and wound tissue isolated from CD-fed, HFD-fed or ob/ob mice. The time after injury is indicated. * as indicated by the brackets. Each single experimental time point depicts the mean ± SD obtained from 12 wounds ( ) isolated from 4 individual animals ( ). (b) Immunoblots showing the presence of F4/80 protein in nonwounded (ctrl) and wounded (1 to 11 d wd) skin in CD, HFD and ob/ob mice as indicated. Each time point depicts 8 wounds from ( ) from 4 individual mice ( ). A Ponceau S (PonS) staining of the immunoblots is shown to control equal loading. (c) Sections from 7-day wound tissue isolated from CD and HFD mice were stained for macrophage-specific F4/80 protein (brown colour) as indicated. F4/80-positive signals were highlighted by arrows. The epithelial margins are indicated by a yellow line. Scale bar = 500 μm. gt, granulation tissue; ne, neoepithelium; sc, scab; we, wound margin epithelia.