Deletion of the Men1 Gene Prevents Streptozotocin-Induced Hyperglycemia in Mice
Figure 1
Ablation of floxed Men1 prevents development of streptozotocin- (STZ-) induced hyperglycemia. (a) and (b) Immunostaining for menin (red) and insulin (green), respectively, in pancreatic islets in mice fed TAM and without STZ treatment. Control and ; Cre-ER mice ( mice) were fed tamoxifen (TAM) at the age of 12 weeks at 200 mg/kg of body weight per day for two consecutive days, followed by one day off and then for another two consecutive days, as described in Section 2. Four weeks after the last dose of TAM feeding, mice were sacrificed and pancreas sections of (a) or ; Cre-ER mice (b) were immunostaed for menin and insulin. (c) A schematic of experimental design. Control ( mice) and ; Cre-ER mice ( mice) were fed TAM at the age of 12 weeks. Four weeks after the last dose of TAM feeding, STZ was i.p. injected at 40 mg/kg of body weight per day for 5 consecutive days. Blood glucose levels were monitored till 4 weeks after STZ injections. (d) and (e) Immunostaining for menin (red) and insulin (green) in islets in control (d) and ; Cre-ER mice (e) that were treated with TAM and followed by STZ injections, as described in Figure 1(c). The pancreata were collected 4 weeks after STZ treatment. (f) Non-fasting blood glucose levels before and until 4 weeks after STZ injections ( to 21 mice). Scale bar, 25 μm. ***, .
