Deletion of the Men1 Gene Prevents Streptozotocin-Induced Hyperglycemia in Mice
Figure 2
Ablation of floxed Men1 promotes beta-cell proliferation and increases beta cell number in STZ-treated mice. Study design was described in Figure 1(c). Control ( mice) and ; Cre-ER mice ( mice) were fed TAM at age of 12 weeks. Four weeks after the last dose of TAM feeding, STZ was i.p. injected at 40 mg/kg of body weight per day for 5 consecutive days. Pancreata and blood serum were collected from mice for further analysis, 4 weeks after STZ injections. (a-b) Immunostaining for insulin (green) and glucagon (red) in islets in control (a) and ; Cre-ER mice (b). Nuclei were counterstained using DABI (blue). (c) Quantitation of number of insulin-secreting beta cells in islets ( mice). (d) Quantitation of percentage of beta cells in islets ( mice). (e) and (f) Immunostaining for BrdU (red) and insulin (green) in islets in control (e) and ; Cre-ER mice (f). (g) Quantitation of BrdU incorporation by beta cells ( mice). (h) Quantitation of insulin staining area ( mice). (i) Non-fasting serum insulin levels before and 4 weeks after STZ injections ( to 21 mice). (j) Ratio of serum insulin (ng/mL) to blood glucose levels (mg/dL), multiplied by 10000. Scale bar, 25 μm. *, ; ***, .
