Deletion of the Men1 Gene Prevents Streptozotocin-Induced Hyperglycemia in Mice
Figure 5
Tissue specific ablation of floxed Men1 promotes beta-cell proliferation and increases beta cell number in STZ-treated mice. Study design was described in Figure 4(e). Control and ; RIP-Cre mice ( mice) at the age of 12 weeks were injected with STZ at 40 mg/kg of body weight per day for 5 consecutive days. Pancreata and blood serum were collected from mice for further analysis, 4 weeks after STZ injections. (a) Quantitation of percentage of insulin-secreting beta cells in islets ( to 7 mice). (b and c) Immunostaining for BrdU (red) and insulin (green) in islets in (b) and ; RIP-Cre mice (c). (d) Quantitation of BrdU incorporation by beta cells ( to 7 mice). (e) Quantitation of insulin staining area ( to 7 mice). (f) Non-fasting serum insulin levels before and 4 weeks after STZ injections ( to 15 mice). Scale bar, 25 μm. *, ; ***, .
