Deletion of the Men1 Gene Prevents Streptozotocin-Induced Hyperglycemia in Mice
Figure 6
Ablation of floxed Men1 preserves membrane localization of glucose transporter 2 (GLUT2) in beta cells in STZ-treated mice. (a-b) Immunostaining for Pdx-1 (red) and insulin (green) in islets in control (a) and ; Cre-ER mice (b) treated with TAM, followed by STZ injections, as described in Figure 1(c). Control ( mice) and ; Cre-ER mice ( mice) were fed TAM at age of 12 weeks. Four weeks after the last dose of TAM feeding, STZ was i.p. injected at 40 mg/kg of body weight per day for 5 consecutive days. Pancreata were collected from mice 4 weeks after STZ injections. Nuclei were counterstained using DABI (blue). (c-d) Immunostaining for GLUT2 (red) and insulin (green) in islets in control (c) and ; Cre-ER mice (d) without STZ treatment, as described in Figures 1(a) and 1(b). Control and ; Cre-ER mice ( mice) were fed tamoxifen (TAM) at the age of 12 weeks at 200 mg/kg of body weight per day for two consecutive days, followed by one day off and then for another two consecutive days. Pancreata were collected 4 weeks after TAM feeding. (e-f) Immunostaining for GLUT2 and insulin in islets in control (e) and ; Cre-ER mice (f) treated with TAM and followed by STZ injections, as described in Figure 1(c). Control ( mice) and ; Cre-ER mice ( mice) were fed TAM at age of 12 weeks. Four weeks after the last dose of TAM feeding, STZ was i.p. injected at 40 mg/kg of body weight per day for 5 consecutive days. Pancreata were collected 4 weeks after STZ injections. Scale bar, 25 μm.
