NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes
Figure 1
MG-HSA increased NF-κB-mediated cytokine production in HK-2 and primary proximal tubular epithelial cells: (a) Cytokines were measured on aliquots of tissue culture supernatant using customized human multiplex kits, using the Bio-Plex system according to the manufacturer’s instructions. Experiments were performed twice in triplicate, and data normalized to control (HK-2 cells exposed to unmodified HSA) values. Data represent mean ± SD of two independent experiments and compared using 1-way ANOVA (significance values are shown within each panel). (b) Renal primary tubular epithelial cells were treated with MG-HSA (5 μM) for various times, and expression levels of IL-6, IL-8, MCP-1, and NIK were measured in a single experiment performed in triplicate (error bars represent SD of intra-assay variability) by using Q-RT-PCR. Data were normalized to GAPDH and expressed as fold change as compared to the untreated cells (Control).
