NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes
Figure 3
DNA-binding assay and cytokine measurements: (a) TransAM NF-κB Family Transcription Factor Assay was used to measure RelA, RelB, and p52 DNA binding following NIKwt and NIKdn overexpression in HK-2 cells. For each Rel protein, ev (left), NIKwt (middle), and NIKdn (right) are shown. Data represent two separate experiments. One-way ANOVA: . (b) Cytokine measurements determined using tissue culture media obtained from the same experiments. Data represent two separate experiments. Two-way ANOVA: . Student’s t-test versus empty vector control: *.
