NF-κB-Inducing Kinase Increases Renal Tubule Epithelial Inflammation Associated with Diabetes
Figure 4
TRAF3 siRNA knockdown: TRAF3 was knocked down using specific siRNA by TransIT-siQUEST Transfection Reagent in HK-2 cells. (a) Representative Western blot demonstrating effects of 100 nM TRAF3 siRNA after 48 and 72 hours with β-actin as a loading control is shown on the left and quantification of the pooled 48 and 72 hour data is shown on the right. All subsequent experiments used 100 nM siRNA. (b) Representative Western blot showing nuclear RelB on the left and quantification of 2 separate experiments on the right; (c) Representative Western blot showing nuclear p52 on the left and quantification of 3 separate experiments on the right; (d) Representative Western blot showing nuclear RelA on the left and quantification of one experiment on the right. Student’s t-test versus empty vector control: *.
