Angiotensin II Type II Receptor Deficiency Accelerates the Development of Nephropathy in Type I Diabetes via Oxidative Stress and ACE2
Figure 5
(a) HO-1 IHC staining (magnification 200X) in the kidneys of 3 subgroups (control, diabetic, and insulin-treated diabetic) of both WT and AT2RKO male mice. (b) HO-1 expression analyzed by Western blot in freshly isolated RPTs from 3 subgroups (control (open bar), diabetic (black bar), and insulin-treated diabetic (shadowed bar)) of WT and AT2RKO male mice. The quantitation of the relative densities of HO-1 normalized to β-actin. The y-axis shows the percentage of relative values compared to WT controls (100%). **P ≤ 0.01; (c) ROS measurement in RPTs. The y-axis shows relative ROS production values compared to WT controls (100%). (d) p47phox mRNA expression in RPTs analyzed by RT-qPCR. Quantitation of p47phox gene was normalized to its own β-actin mRNA. The y-axis shows the percentage of relative values compared to WT animals (100%). *P ≤ 0.05. **P ≤ 0.01; NS, nonsignificant.
