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Experimental Diabetes Research
Volume 2012, Article ID 128694, 9 pages
Research Article

Decreased Skin-Mediated Detoxification Contributes to Oxidative Stress and Insulin Resistance

1Department of Physiology, Medical College, Dalian University, Dalian 116622, China
2Department of Histology and Embryology, Medical College, Dalian University, Dalian 116622, China
3Department of Pathology, Xinxiang Medical College, Xinxiang 453003, China
4Department of Physiology, China Medical University, Shenyang 110001, China
5College of Environmental and Chemical Engineering, Dalian University, Dalian 116622, China

Received 21 February 2012; Revised 28 May 2012; Accepted 12 June 2012

Academic Editor: Pietro Galassetti

Copyright © 2012 Xing-Xing Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The skin, the body's largest organ, plays an important role in the biotransformation/detoxification and elimination of xenobiotics and endogenous toxic substances, but its role in oxidative stress and insulin resistance is unclear. We investigated the relationship between skin detoxification and oxidative stress/insulin resistance by examining burn-induced changes in nicotinamide degradation. Rats were divided into four groups: sham-operated, sham-nicotinamide, burn, and burn-nicotinamide. Rats received an intraperitoneal glucose injection (2 g/kg) with (sham-nicotinamide and burn-nicotinamide groups) or without (sham-operated and burn groups) coadministration of nicotinamide (100 mg/kg). The results showed that the mRNA of all detoxification-related enzymes tested was detected in sham-operated skin but not in burned skin. The clearance of nicotinamide and N1-methylnicotinamide in burned rats was significantly decreased compared with that in sham-operated rats. After glucose loading, burn group showed significantly higher plasma insulin levels with a lower muscle glycogen level than that of sham-operated and sham-nicotinamide groups, although there were no significant differences in blood glucose levels over time between groups. More profound changes in plasma H2O2 and insulin levels were observed in burn-nicotinamide group. It may be concluded that decreased skin detoxification may increase the risk for oxidative stress and insulin resistance.