Differential Proteome Profiling Using iTRAQ in Microalbuminuric and Normoalbuminuric Type 2 Diabetic Patients

Figure 6

Validation using Western blot for six representative differentially excreted proteins. The concentrations of transferrin (a), ceruloplasmin (b), α1-antitrypsin (c), vitamin D-binding protein (d), α1-acid glycoprotein (e), and haptoglobin (f) are significantly higher in microalbuminuric patients versus normoalbuminuric urine. The relative intensities on the vertical axis indicate normalized values versus the representative control. Each bar represents the mean ± SEM, based on the relative intensities of the gel bands. Statistical significance for the differences (*𝑃<0.05; **𝑃<0.01; ***𝑃<0.005; ****𝑃<0.0005) were determined by paired Student’s t-test. Two representative NA and MA blots are shown at the top of the bar graphs.

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