Rising Intracellular Zinc by Membrane Depolarization and Glucose in Insulin-Secreting Clonal HIT-T15 Beta Cells
Figure 3
Effect of extracellular calcium or zinc on KCl induced intracellular zinc rise. (a) KCl (60 mM) induced intracellular zinc rises in the presence or absence of extracellular calcium. The latter was achieved by bathing cells with “no calcium” medium (no calcium addition) or “calcium-free medium” (no calcium addition plus 500 μM EGTA). (b) KCl (60 mM) induced intracellular zinc rise in the control medium and the medium containing 1 mM CaEDTA to remove extracellular zinc. In (a) and (b), each experiment was done on separate set of cells. (c) Comparison of half time in seconds to reach the maximum stimulation induced by KCl alone () and KCl with 1 mM CaEDTA (), . Each point represents the averaged, normalized zinc rise initial slope ± SEM from data in (b).
