L(+) and D(-) Lactate Are Increased in Plasma and Urine Samples of Type 2 Diabetes as Measured by a Simultaneous Quantification of L(+) and D(-) Lactate by Reversed-Phase Liquid Chromatography Tandem Mass Spectrometry
Table 2
Linearity tested in different matrices.
Matrix
Slope(a)
-intercept
Concentration range (μmol/L)
D-lactate
Water
1.2927
−4.5
0.9971
0–105
Plasma A
1.4307
21.2
0.9987
0–105
Plasma B
1.3820
51.3
0.9991
0–105
Plasma C
1.5037
31.9
0.9990
0–105
Mean
1.4023
CV(%)
6.3
L-lactate
Water
0.7768
−4.9
0.9996
0–3008
Plasma A
0.7566
1032
0.9998
0–6016
Plasma B
0.7164
996
0.9997
0–6016
Plasma C
0.7534
1346
0.9997
0–3008
Mean
0.7502
CV(%)
3.2
D-lactate
Water
1.1265
−15.4
0.9999
0–702
Urine A
1.1092
105.24
0.9984
0–351
Urine B
1.1114
99.7
0.9999
0–702
Urine C
1.0287
69.8
0.9992
0–351
Mean
1.094
CV(%)
4.04
L-lactate
Water
0.7967
0.2
0.9998
0–1002
Urine A
0.8039
60.4
0.9995
0–501
Urine B
0.7918
48.6
0.9995
0–1002
Urine C
0.7749
135.0
0.9993
0–501
Mean
0.7967
CV(%)
1.56
(a)Slope: {concentration (μmol/L)} versus {response = (peak area component/peak area internal standard). *Internal standard concentration (μmol/L)}.
