Animal Models of Diabetes Mellitus for Islet Transplantation
Figure 1
STZ injection via intraperitoneum. (a) Preparation. 1.5 mL centrifuge tube with STZ, 29-gauge needle, 1 mL syringe, ice box with crushed ice, citrate buffer solution, and an 18-gauge needle are used. (b)–(e) Dissolving STZ. (b) Citrate buffer solution is poured into STZ. (c) Crushing the larger clusters of STZ with an 18-gauge needle. (d) and (e) Dissolving by shaking gently. (f) STZ solution is injected via the lower abdomen with care not to injure organs.