Metformin Stimulates FGF21 Expression in Primary Hepatocytes

Figure 1

Dose-dependent effect of metformin and AICAR on FGF21 expression. (a) FGF21 mRNA and (b) protein expression in primary rat hepatocytes incubated with metformin for 24 hrs. (c) FGF21 mRNA expression in primary human hepatocytes incubated with metformin for 24 hrs. (d) FGF21 mRNA expression in primary rat hepatocytes incubated with AICAR for 24 hrs. Data are means ± SEM; ^∗𝑃<0.05, ^∗∗𝑃<0.01, ^∗∗∗𝑃<0.0001 versus nontreated hepatocytes, analyzed by paired Student’s t-test, 𝑛= 4–8.

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