Figure 4: AMPK phosphorylation is stimulated by metformin. Level of (a, b) phosphorylated AMPKα normalized to protein content, and (c) phosphoACC detected by Western blotting, in primary rat hepatocytes. The hepatocytes were incubated for 24 hrs with (a) metformin or (b, c) Compound C in the presence of 1000 μM metformin. Data are means ± SEM; , versus nontreated hepatocytes, versus 0 μM Compound C treated hepatocytes, analyzed by paired Student’s t-test, -5.