A PEDF-Derived Peptide Inhibits Retinal Neovascularization and Blocks Mobilization of Bone Marrow-Derived Endothelial Progenitor Cells
Figure 2
Antiangiogenic activity of PEDF 34-mer during in vivo blood vessel formation. CAMs were separately treated with either 50 or 100 nM PEDF-34. (a) Left: close-up image of a chicken embryo CAM with a nitrocellulose disk containing PEDF-34 (100 nM). Middle: the disk area after removal of PEDF-34 nitrocellulose disk. Right: image of a PBS-treated-nitrocellulose control area on the same CAM with the disk removed. Note considerable reduction in the overall number of small newly formed blood vessels, when compared with the PBS-treated control. (b) Blood vessel density in the CAMs was assessed by software analysis. Vascular densities in the disk areas of CAMs treated with 50 and 100 nM were quantified and averaged. The graph represents the percent of vascular density found under PEDF-34-treated areas compared to the PBS-treated control area, which was set at 100%. Vascular density in the CAM treated with PEDF-34 is significantly lower than the control (mean + SD, ).
