Regulation of Pancreatic microRNA-7 Expression

Figure 1

Three miR-7 genes are expressed in beta cells. (a) and (b) Taqman qPCR analysis for mature miR-7a and miR-7b in several tissues: the aorta and pulmonary artery, heart, isolated islets, and brains from three months old mice. Data normalized to sno234. (c) qPCR analysis for miR-7 precursors reveals expression in beta-TC cultured cells. Data normalized to GAPDH and 18S. 𝑛=4 independent measurements, duplicates each. (d) Taqman qPCR analysis to mature miR-7a and miR-7b in beta-TC cultured cells. Data normalized to sno234. 𝑛=4 independent measurements in duplicates. Error bars represent ± SEM.

(a)

(b)

(c)

(d)