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Experimental Diabetes Research
Volume 2012, Article ID 721256, 8 pages
http://dx.doi.org/10.1155/2012/721256
Research Article

Beneficial Effects of Aminoguanidine on Skin Flap Survival in Diabetic Rats

1Department of Plastic Reconstructive and Aesthetic Surgery, Inonu University School of Medicine, 44380 Malatya, Turkey
2Department of Plastic, Reconstructive and Aesthetic Surgery, Turgut Ozal Medical Center, Inonu University School of Medicine, 44380 Malatya, Turkey
3Department of Pharmacology, Inonu University School of Medicine, 44380 Malatya, Turkey
4Department of Biochemistry, Inonu University School of Medicine, 44380 Malatya, Turkey
5Department of Pathology, Inonu University School of Medicine, 44380 Malatya, Turkey

Received 12 July 2012; Revised 13 November 2012; Accepted 27 November 2012

Academic Editor: T. S. Kern

Copyright © 2012 Ayse Ozturk et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Random flaps in DM patients have poor reliability for wound coverage, and flap loss remains a complex challenge. The protective effects of aminoguanidine (AG) administration on the survival of dorsal random flaps and oxidative stress were studied in diabetic rats. Two months after the onset of DM, dorsal McFarlane flaps were raised. Forty rats were divided into four groups: (1) control, (2) AG, (3) DM, and (4) DM + AG groups. Flap viability, determined with the planimetric method, and free-radical measurements were investigated. In addition, HbA1c and blood glucose levels, body weight measurements, and histopathological examinations were evaluated. The mean flap necrotic areas (%) in Groups I to IV were 50.9 ± 13.0, 32.9 ± 12.5, 65.2 ± 11.5, and 43.5 ± 14.7, respectively. The malondialdehyde (MDA) and nitric oxide (NO) levels were higher in the DM group than in the nondiabetic group, while the reduced glutathione (GSH) levels and superoxide dismutase (SOD) activity were reduced as a result of flap injury. In the diabetic and nondiabetic groups, AG administration significantly reduced the MDA and NO levels and significantly increased GSH content and SOD enzyme activity. We concluded that AG plays an important role in preventing random pattern flap necrosis.