Effect of propionate treatment (0.3 g/kg, i.p. for 7 days) on Akt knockout-induced change in pan and phosphorylated GSK3β, eNOS, GPR41, and PGR43 levels. (a) pGSK3β-to-GSK3β ratio; (b) eNOS expression; (c) GPR41 expression; (d) GPR43 expression. Insets: representative gel blots depicting expression and/or phosphorylation of these proteins using specific antibodies. β-Actin was used as the loading control. Mean ± SEM, mice per group, *P < 0.05 versus WT group, and #P < 0.05 versus AKTKO group.
