A Comparison of Inflammatory and Oxidative Stress Markers in Adipose Tissue from Weight-Matched Obese Male and Female Mice
Figure 4
Relative mRNA expression of markers for immune cell infiltration and oxidative stress is decreased in gonadal adipose tissue isolated from obese female mice as compared to obese male mice. Male and female C57BL/6 mice were fed either a standard rodent chow or a high-fat diet (HFD) from 6 weeks of age until the HFD-fed group achieved a body weight of 45 g. At that time, both chow- and HFD-fed males (21 weeks old) or females (38 weeks old) were sacrificed and qRT PCR was performed on gonadal adipose tissue. Relative mRNA expression of markers for immune cell infiltration and inflammation (a) as well as oxidative stress (b) was determined. Data are reported as mean ± SE; –8 per group; means with different superscripts differ by . IL-6: interleukin-6; MCP-1: monocyte chemoattractant protein-1; TNF-α: tumor necrosis factor-alpha; eNOS: endothelial nitric oxide synthase; iNOS: inducible nitric oxide synthase; HO-1: heme oxygenase-1; p40phox: NADPH subunit p40phox; Prdx1: peroxiredoxin-1.
