Research Article

Transcriptional Mechanisms Controlling miR-375 Gene Expression in the Pancreas

Figure 4

Characterization of the inhibitory effect of blocks 3 and 4. (a) Representation of plasmids containing blocks 3 and 4 inserted in both orientations upstream to the CMV-TK promoter, controlling the firefly luciferase reporter gene. (b) Luciferase activity was measured following transfection into the beta cell lines MIN6 and HIT, the alpha cell line αTC1, and the non-pancreatic cell line NIH-3T3. Activity is expressed relative to luciferase activity of the promoter-less vector pGL3-basic. Each data point represents mean ± SEM ( ; * , as compared to the activity of CMV-TK promoter).
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