The Role of Adrenomedullin in the Renal NADPH Oxidase and (Pro)renin in Diabetic Mice

Figure 3

Renin-secreting granular cells in the juxtaglomerular apparatus and their correlation with the NADPH oxidase expression in the macula densa. (a) PAM staining illustrating granular cells in the juxtaglomerular apparatus with silver stained renin granules and reddish cytoplasm. The bar indicates 50 𝜇m. (b) The granular cell (GC) hyperplasia scores were increased in the STZ-induced diabetic wild mice (Wild-DM) and the adrenomedullin gene knockout (AMKO) mice (AMKO-DM) compared with those observed in the control mice (Wild-C and AMKO-C). The AMKO-DM mice exhibited further increases in granular cells compared with the Wild-DM mice. Four mice in each group. ^∗𝑃<0.005, ^∗∗𝑃<0.0001 versus Wild-C, ⁺𝑃<0.001 versus AMKO-C, and ^#𝑃<0.01 versus Wild-DM. (c) The correlation between the renin-secreting granular cells (GC) hyperplasia score in the juxtaglomerular apparatus and the NADPH oxidase p47phox immunoreactivity score in the macula densa. The white circles indicate wild-control mice, the black circles indicate diabetic wild mice, the white triangles indicate adrenomedullin gene knockout (AMKO)-control mice, and the black triangles indicate diabetic AMKO mice. 𝑅=0.96, 𝑃<0.03.

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