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Journal of Diabetes Research
Volume 2013, Article ID 185172, 9 pages
http://dx.doi.org/10.1155/2013/185172
Research Article

Protective Effects of Resveratrol on TNF- -Induced Endothelial Cytotoxicity in Baboon Femoral Arterial Endothelial Cells

1Department of Endocrinology, Qilu Hospital, Shandong University, 107 Wen Hua Xi Lu, Jinan, Shandong 250012, China
2Southwest National Primate Research Center, Texas Biomedical Research Institute, P.O. Box 760549, San Antonio, TX 78245-0549, USA
3Cardiothoracic Research Laboratory, Texas Heart Institute, Baylor College of Medicine, Houston, TX 77030-2604, USA

Received 5 January 2013; Revised 27 February 2013; Accepted 1 March 2013

Academic Editor: Weiping Jia

Copyright © 2013 Juan Xiao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Endothelial injury induced by inflammatory factors plays a critical role in the pathogenesis of cardiovascular disease. Endothelial cell (EC) apoptosis, proliferation, migration, and cellular adhesion molecule (CAM) expression contribute to the development of atherosclerosis. We investigated the effects of resveratrol (0.1–100 μM) on the proliferation, migration, and CAM expression of primary cultures of baboon arterial endothelial cells (BAECs). In addition, we tested its effects under normal conditions as well as under inflammatory conditions induced by tumour necrosis factor- (TNF- ) administered either by cotreatment, pretreatment, or posttreatment. Immunocytochemistry, MTT, wound-healing, and flow cytometry assays were performed. The resveratrol treatment significantly enhanced BAEC proliferation and attenuated TNF- -induced impairment of proliferation at the optimal doses of 1–50 µM. Resveratrol at a high dose (100 μM) and TNF- impaired BAEC migration, while low doses of resveratrol (1–50 μM) attenuated TNF- -induced impairment of BAEC migration. Moreover, resveratrol inhibited TNF- -induced ICAM-1 and VCAM-1 expression. Taken together, our results suggest that the resveratrol protects BAECs after inflammatory stimulation as well as ameliorates inflammatory effects at low concentrations. Consequently, resveratrol should be considered as a candidate drug for the prevention and treatment of inflammatory vascular diseases.