Outcome of Acute Renal Injury in Diabetic Mice with Experimental Endotoxemia: Role of Hypoxia-Inducible Factor-1α
Figure 5
(a) Western-blotting analysis of HIF-1α in renal tissue. Quantification of HIF-1 α signal normalized by β-actin signal is also shown. (b) HIF-1α immunohistochemistry. I: control kidney. An intense immunoexpression of HIF-1α was detected in the cytoplasm of proximal convoluted tubules (*), and in the nuclei of distal tubules, the labelling was weaker (). II: diabetic mice. Proximal tubules showed positive reaction to HIF-1α in the cytoplasm (■). Both nucleus and cytoplasm of distal convoluted tubules cells (▲) presented a positive reaction to HIF-1α. The arrow points to the urinary pole with moderate reaction to this antibody. III: LPS-treated mice. Both convoluted tubules showed a strong immunolabelling to HIF-1α. IV: diabetic LPS-treated mice. The highest reaction to HIF-1α was encountered in the kidney of diabetic mice treated with endotoxin. Magnification: I and II: ×600; III and IV: ×300.