Pronerve Growth Factor Induces Angiogenesis via Activation of TrkA: Possible Role in Proliferative Diabetic Retinopathy
Figure 6
Inhibiting TrkA prevents proNGF-mediated retinal endothelial cell migration. BRE cells were grown to confluence and then scratched using a standard cell scrapper. Cells were switched to serum free medium and treated with mutant proNGF (50 ng/mL) in the presence or absence of either TrkA receptor antagonist, K252a (0.1 μM), or p75 inhibitor (20 μM). Representative micrographs for wounded BRE cells are shown after 18 hours of various treatments. Statistical analysis showed that proNGF increased mean cell migration by 1.98-fold compared to the control group. This effect was blocked by the specific TrkA receptor antagonist (K252a) but not with p75 inhibitor (*,‡statistically significant compared to control and proNGF groups, resp., (), ).
