Impact of High Glucose and Proteasome Inhibitor MG132 on Histone H2A and H2B Ubiquitination in Rat Glomerular Mesangial Cells

Figure 3

The expression of histone H2A and H2B ubiquitination in each group of GMCs by Western blot (48 hr). (a) uH2A and uH2B proteins at different glucose concentrations and high osmotic pressure at 48 hr: uH2A increased in the high glucose group and uH2B decreased as the glucose concentrations increased; they had the most significant changes in the HG3 group, but there were no apparent differences between the NC group and the OP group. (b) The gray graph shows the relative statistical values of uH2A for each group. The expression of the uH2A increased in the high glucose group, especially, in the HG3 group. ^∗𝑃<0.01 versus NC group, ^#𝑃<0.03 versus HG1 group, and ^※𝑃<0.05 versus HG2 group. (c) The gray graph shows the relative statistical values of uH2B for each group. The expression of the uH2B proteins decreased by concentration dependency, obviously in HG3 group. ^∗𝑃<0.01 versus NC group, ^#𝑃<0.03 versus HG1 group, and ^※𝑃<0.05 versus HG2 group.

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