Research Article

Impact of High Glucose and Proteasome Inhibitor MG132 on Histone H2A and H2B Ubiquitination in Rat Glomerular Mesangial Cells

Figure 5

The expression of histone H2A and H2B ubiquitination after intervention with MG132 determined using Western blot (uH2A for 24 hr and uH2B for 48 hr). (a) Western blot strip chart. (b) The gray graph shows the relative statistical values of uH2A for each group. Compared with the NC group, the expression of the uH2A protein was significantly increased in the HG3 group. After MG132 intervention, the protein expression of uH2A decreased. versus NC group and versus 1 HG3 group. (c) The gray graph shows the relative statistical values of uH2B for each group. Compared with the NC group, the expression of the uH2B protein was significantly decreased in the HG3 group. And after MG132 intervention, uH2B relative to the normal group changed. versus NC group and versus 1 HG3 group.
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